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KMID : 1190620050020010028
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Journal of the Korean Wound Care Society
2005 Volume.2 No. 1 p.28 ~ p.33
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Co-supplementation Effect of GM-CSF and Vitamin-C on Wound Healing In Vitro
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Hong Yong-Taek
Han Seung-Kyu
Kim Jeong-Bae
Kim Woo-Kyung
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Abstract
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Granulocyte-macrophage colony-stimulating factor (GM-CSF) is naturally occuring protein that has the healing effect on normal wound as well as infected nonhealing wounds and ulcers. Vitamin-C (ascorbic acid) has also been shown to stimulate fibroblast stimulation and collagen synthesis. However, there are no reports about the effect of GM-CSF and ascorbic acid co-supplementation on wound healing. Therefore, in this report, we examined the effect of GM-CSF and ascorbic acid co-supplementation on the proliferation of human dermal fibroblasts and collagen synthesis which play a crucial role in wound healing process in vitro. To determine an optimal GM-CSF and ascorbic acid concentration for human dermal fibroblast proliferation and collagen synthesis, the cells were incubated with varying concentrations of GM-CSF and ascorbic acid. The activity of fibroblast proliferation was determined by MTT assay. To measure the amount of collagen production, the collagen type I carboxy-terminal propeptide enzyme immunoassay was used. The best fibroblast proliferation was seen at the co-supplementation of 0.5μg/ml GM-CSF with 25μg/ml ascorbic acid and 1μg/ml GM-CSF with 12.5μg/ml ascorbic acid. Maximal stimulation of collagen synthesis was seen at the co- supplementation of 1μg/ml GM-CSF with 12.5μg/ml ascorbic acid. The collagen synthesis per cell was also maximal at concentration of 1μg/ml GM-CSF with 12.5μg/ml ascorbic acid. This results demonstrated that GM-CSF and ascorbic acid co-supplementation increased human dermal fibroblast proliferation and collagen synthesis and the concentration was critically important factor in vitro. (J Korean Wound Care Soc 2006;2:28-33)
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KEYWORD
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GM-CSF, Vitamin-C
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